Perform research to study transcription and/or DNA properties that affect transcription and its regulation using techniques the best suited single molecule techniques available in the lab, which span from TPM, atomic force microscopy (AFM), magnetic tweezers and the C-trap microscope. Acquisition of data and data analysis. Draft manuscript describing results. Other duties as assigned
JOB DUTIES: 40% - Essential - Use single molecule techniques to investigate DNA transactions, including (but not necessarily limited to) transcription, compaction by regulatory proteins, conformational changes as a function of the environment. These investigations involve preparation of (i) microchambers to look at samples under the microscope, (ii) DNA-protein samples, (iii) manipulation of DNA-protein complexes applying torsional and/or tensile forces, (iv) data acquisition and analysis, (v) preparation of figures summarizing the experimental data collected, (vi) limited coding, (vii) writing drafts of paper and (viii) possibly presenting results at meetings.
20% - Essential - Prepare samples needed for single molecule microscopy. These preparations involve the assembly of microscope chambers from slides and coverslips, preparation of buffers and basic molecular biology such as pcr, restriction and ligation reactions.
20% - Essential - Acquisition of data and data analysis. This may involve recording of AFM images, single particle tracking in TPM, magnetic and optical tweezers and/or quantification of fluorescence intensity in the C-Trap. Data analysis will involve plotting of data, applying physics and mathematical modeling, with possible tuning of existing codes or writing of new algorythms and interpretation of the results.
20% - Essential - Draft manuscript describing results will involve preparing figures and writing text that introduces the significance of the research, describes the materials and methods, describes the results and discusses their meaning.
MINIMUM REQUIREMENTS: Education - Doctoral / Professional Degree
Experience: Research at the doctoral and postdoctoral level, single molecule expertise
RESPONSIBILITIES JOB KNOWLEDGE
Firm Job Knowledge - Firm working knowledge of concepts, practices and procedures and ability to use in varied situations
SUPERVISORY RESPONSIBILITIES
No Supervisory Duties - Not responsible for supervising employees.
BUDGETARY RESPONSIBILITIES
No Budget Responsibilities - No fiscal responsibility for the department's budget.
PHYSICAL REQUIREMENTS: 40% - Sit (stationary position) for prolonged period
60% - Walk or move about
60% - Communicate, converse, give direction, express oneself
30% - Recognize or inspect visually
WORKING CONDITIONS: No Work Conditions
WORK SCHEDULE: Standard Hours: 37.5
COMPENSATION INFORMATION Expected Salary Range: $42,800-$60,000
Salary is dependent upon several factors including, but not limited to, a candidate's previous experience, knowledge, skills and performance in accordance with Clemson's compensation guidelines.
ESSENTIAL PERSONNEL LEVEL Normal Operations
Required to follow emergency facility closure directives, and not normally expected work on-site during emergency situations.
JOB LOCATION: Clemson, SC
APPLICATION DEADLINE: May 20, 2024
MILITARY AND VETERAN: MILITARY EQUIVALENCY:
Clemson University is proud to allow educational equivalency for military technical certifications and trainings that directly relate to the job duties.
VETERAN PREFERENCE:
South Carolina is making our Veterans a priority for employment in state agencies and institutions.
State policy for veteran preference states that for qualifying, full-time permanent positions, a veteran applicant may receive preference if they meet the job's minimum qualifications, were discharged under honorable conditions from the military, and submit their DD-214 for confidential review by the Office of Human Resources.
To claim Veteran Preference for qualifying positions, email upon submission of your application.
CLOSING STATEMENT: Clemson University is an AA/EEO employer and does not discriminate against any person or group on the basis of age, color, disability, gender, pregnancy, national origin, race, religion, sexual orientation, veteran status or genetic information.
